You have been given a 1.0 kbp human genome fragment that does not contain any repeated sequences. Your plan is to use it as a probe for a Southern…

You have been given a 1.0 kbp human genome fragment that does not contain any repeated sequences. Your plan is to use it as a probe for a Southern Blot containing your friend’s genomic DNA. You prepare the gel by digesting with the following restriction enzymes in the following lanes:

Lane 1: size marker

Lane 2: EcoRI

Lane 3: HindIII

Lane 4: BamHI

Your friend happens to be homozygous for the region you are probing. Does that mean you will only see one band in each lane? 

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